Further in silico analysis of CaM sequences reveals variation in EF-hand position when these sequences were subjected to various online bioinformatics tool viz. Structures of all twelve cloned CaMs were modeled using MOE employing the NMR structure of CaM (PDB accession no. 2F3Y.A) as a template and obtained a refined model after energy minimization. Ann Bot 92: 487-511. Yang T, Poovaiah BW (2003) Calcium/calmodulin-mediated signal network in plants.